Effect of Local Administration of Laminin and Fibronectin with Chitosan Conduit on Peripheral Nerve Regeneration: A Rat Sciatic Nerve Transection Model
Authors
Abstract:
Objective-Effect of local administration of laminin and fibronectin on nerve regeneration was assessed. Design- Experimental study. Animal- Sixty male Wistar rats. Procedures- The animals were divided into four experimental groups (n=15), randomly: In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group (CHIT/LF) the defect was bridged using a chitosan conduit filled with 10 µL mixture at a concentration of 1 mg/ml laminin and 1 mg/ml fibronectin in a 1:1 volumetric addition. In CHIT group the conduit was filled with phosphate-buffered saline. In normal control group sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and nerve fibers were studied in a 12-week period. Results- Behavioral, functional, gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in CHIT/LF than in CHIT group (p<0.05). Immunohistochemical reactions to S-100 in CHIT/LF were more positive than that in CHIT group. Conclusion and Clinical Relevance- Laminin-fibronectin improved functional recovery and morphometric indices of sciatic nerve. They could be considered as an effective treatment for peripheral nerve repair in practice.
similar resources
Effect of Local Administration of Brain Derived Neurotrophic Factor with Silicone Conduit on Peripheral Nerve Regeneration: a Rat Sciatic Nerve Transection Model
Objective- The objective was to assess local effect of brain derived neurotrophic factor (BDNF) on functional recovery of peripheral nerve in rat sciatic nerve transection model. Design- Experimental study. Animals- Sixty male healthy white Wistar rats Procedures- The animalswere randomized into four experimental groups of 15 animals each: In sham-operated group (SHAM), sciatic nerve was exp...
full textUse of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat
Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat Design- Experimental in vivo study. Animals- Sixty healthy male Wistar rats. Procedures-The rats were divided into four experimental groups (n=15) randomly. In sham group the left sciatic nerve was exposed through a gluteal muscle incision and after careful...
full textUse of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat
Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat DesignExperimental in vivo study. AnimalsSixty healthy male Wistar rats. Procedures-The rats were divided into four experimental groups (n=15) randomly. In sham group the left sciatic nerve was exposed through a gluteal muscle incision and after careful homeostasis the...
full texteffect of local administration of platelet-derived growth factor b on functional recovery of peripheral nerve regeneration: a sciatic nerve transection model
background: effects of platelet-derived growth factor b (pdgf-b) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model. materials and methods: forty‑five male, white wistar rats were divided into three experimental groups (n = 15), randomly: normal control group (nc), silicon group (sil), and pdgf‑b treated group (sil/pdgf). in nc group, left sciatic nerve was...
full textHomeopathic treatment for peripheral nerve regeneration: an experimental study in a rat sciatic nerve transection model.
AIM Effects of homeopathic treatment with Hypericum perforatum (Hypericum) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model. METHODS Fifty-four male healthy White Wistar rats were divided into three experimental groups (n = 18), randomly: Sham-operation (Sham), control: silicon tube (Sil) and treatment: silicon tube + Hypericum (Sil/Hypericum). In the S...
full textAlpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat
Objective(s): To investigate the effect of topical administration of alpha-lipoic acid into chitosan conduit on peripheral nerve regeneration using a rat sciatic nerve transection model. Materials and Methods: Forty five Wistar rats were divided into three experimental groups randomly. A 10-mm gap of sciatic nerve was bridged with a chitosan conduit following surgical preparation and anesthesia...
full textMy Resources
Journal title
volume 10 issue 2
pages 39- 47
publication date 2015-12-01
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023